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Literature summary for 4.2.1.93 extracted from

  • Colinas, M.; Shaw, H.; Loubery, S.; Kaufmann, M.; Moulin, M.; Fitzpatrick, T.
    A pathway for repair of NAD(P)H in plants (2014), J. Biol. Chem., 289, 14692-14706 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.2.1.93

Cloned(Commentary)

Cloned (Comment) Organism
gene At5g19150, sequence comparisons, functional recombinant expression of YFP-tagged enzyme in wild-type Arabidopsis thaliana leaf mesophyll via Agrobacterium tumefaciens strain C58 transformation, enzyme expression analyses, functional recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli, coexpression with NNRE or PDX3 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information the nnrd-1 mutant line GK-104E02, phenotype, overview. NNRD is dispensable for growth and development under standard conditions Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma
-
 Arabidopsis thaliana 9570
-
mitochondrion
-
 Arabidopsis thaliana 5739
-
additional information NNRD has multiple locations, prediction of an N-terminal transit peptides for NNRD with a probability of mitochondrial and plastid localization for NNRD, the recombinant YFP-tagged enzyme from leaf mesophyll shows localization in cytosol, chloroplast stroma, and mitochondria, overview. NNRD is predominantly (if not exclusively) found in chloroplasts and mitochondria Arabidopsis thaliana
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
132700
-
 recombinant enzyme, gel filtration Arabidopsis thaliana
142800
-
 sequence calculation, about Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana
-
 ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide Arabidopsis thaliana Col-0
-
 ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana
-
 ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate Arabidopsis thaliana Col-0
-
 ADP + phosphate + NADPH
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q94AF2
-
-
Arabidopsis thaliana Col-0 Q94AF2
-
-

Purification (Commentary)

Purification (Comment) Organism
preparation of mesophyll protoplasts from stable Arabidopsis thaliana transfection lines recombinantly expressing the enzyme, isolation of mitochondria from transgenic seedlings or protoplasts, recombinant C-terminally His6-tagged enzyme from Escherichia coli by ncikel affinity chromatography. No copurification with coexpressed NNRE or PDX3 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
seed NNRD is restricted to seeds but appears to be dispensable during the normal Arabidopsis life cycle, NNRD accumulates in seeds Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Arabidopsis thaliana ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide
-
 Arabidopsis thaliana Col-0 ADP + phosphate + NADH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Arabidopsis thaliana ADP + phosphate + NADPH
-
 ?
ATP + (6S)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
-
 Arabidopsis thaliana Col-0 ADP + phosphate + NADPH
-
 ?
additional information the dehydratase is stereospecific and only uses (S)-NADHX. Coupled enzyme assay with the NADHX epimerase from Arabidopsis thaliana Arabidopsis thaliana ?
-
 ?
additional information the dehydratase is stereospecific and only uses (S)-NADHX. Coupled enzyme assay with the NADHX epimerase from Arabidopsis thaliana Arabidopsis thaliana Col-0 ?
-
 ?

Subunits

Subunits Comment Organism
tetramer
-
 Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
At5g19150
-
 Arabidopsis thaliana
NAD(P)HX dehydratase
-
 Arabidopsis thaliana
NNRD
-
 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
ATP dependent on Arabidopsis thaliana

General Information

General Information Comment Organism
evolution the enzyme belongs to the ribokinase superfamily Arabidopsis thaliana
additional information Arabidopsis thaliana NNRD and NNRE function in non-stoichiometric oligomeric states. Homology models of the three-dimensional structure of NNRD from Arabidopsis thaliana Arabidopsis thaliana
physiological function a stereospecific dehydratase (NNRD) and an epimerase (NNRE) constitute the NAD(P)H repair pathway and demonstrate their activity as NAD(P)HX repair enzymes. the ATP-dependent NNRD and NNRE act concomitantly to restore NAD(P)HX to NAD(P)H, but the proteins do not physically interact. The epimerase acts in conjunction with the dehydratase converting (R)-NAD(P)HX into (S)-NAD(P)HX. Whereas NNRE is present ubiquitously, NNRD is restricted to seeds but appears to be dispensable during the normal Arabidopsis life cycle Arabidopsis thaliana